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EQUINE MEDICATION AND METABOLITE REFERENCE
STANDARDS AND STABLE ISOTOPE STANDARDS
Sucheta Kudrimoti, Julio Gutierrez, Wojtek Karpiesiuk, Elizabeth Armstong,
Charlie Hughes, Job Tharappel, and Thomas Tobin.
The Maxwell H. Gluck Equine Research Center,
Department of Veterinary Science,
University of Kentucky, Lexington, KY 40546
Rodney Eisenberg.
Frontier Biopharm, PO Box 614, Richmond, KY 40476
www.frontierbiopharm.com
Brent Mayer and Emilie Stanley.
Neogen Corp., Nandino Boulevard, Lexington, KY 40511
The equine medication metabolite standards and stable isotope reference standards listed
here were synthesized by a team of scientists with headquarters in the
University of Kentucky Gluck Equine Research Center. These syntheses have been an ongoing project, starting in the mid-nineties
when the need was recognized for standards of unique equine drug metabolites. More recently, there has been an increasing need for high-quality certified reference standards and stable isotope internal standards for use in quantitative equine medication regulation, or more simply, to allow the regulatory application of thresholds for therapeutic
medications.
The University of Kentucky and Frontier Biopharm, in cooperation with Neogen Corp., are working to provide the
racehorse drug testing community with certified quantitative analytical reference standards for equine therapeutic medications that will satisfy the ISO-17025 and ISO-34 requirements for quantitative measurements, and related reference materials which are increasingly required for equine forensic testing. These reference standards will meet industry requirements for chemical
identity and purity in accordance with accepted ISO analytical criteria.
Synthesis of these standards has been made possible by ongoing research support from a large number of Horsemen’s Benevolent and Protective Association’s, starting with the Florida HBPA in the mid-90s, and since then including the National Horsemen’s Benevolent and Protective Association and the Alabama, Arizona, Arkansas, Canada, Charles Town West Virginia, Florida, Iowa, Indiana, Kentucky, Louisiana, Michigan, Minnesota, Nebraska, Ohio, Oklahoma, Ontario Canada, Oregon, Pennsylvania, Tampa Bay Downs Florida, Texas, Washington State, and West Virginia Horsemen’s Benevolent and Protective Associations, the Kentucky Equine Drug Research Council, and the Kentucky Racing Commission, the Kentucky Horse Racing Authority
and the Kentucky Science and Engineering Foundation, Grant agreement KSEF 148-502-05-160 with the Kentucky Science and Technology Corporation, and this support is gratefully acknowledged, along with the ongoing support of the Director and Faculty of the Maxwell H. Gluck Equine Research Center and the University of Kentucky College of Agriculture.
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Available Standards
--- follow the
links to view PDF certificates of analysis ---
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Parent Medication
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Product
Number
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Regulatory
Analyte or
Deuterated Internal Standard
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Acepromazine
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H-1002
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(HEPS-d4)
2-(1-hydroxyethyl)
promazine sulfoxide-d4 maleate
(deuterated metabolite)
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Acepromazine
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H-1001
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(HEPS)
2-(1-hydroxyethyl)
promazine sulfoxide maleate
(metabolite)
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Bupivacaine
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BU-2950
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3-Hydroxybupivacaine
(metabolite)
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Clenbuterol
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Clen-009
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Clenbuterol-d9
(deuterated standard)
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Detomidine
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C-1001
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Carboxydetomidine
(metabolite)
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Detomidine
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C-5001
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3-Carboxydetomidine-d4
(deuterated metabolite)
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Detomidine
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H-2120
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Hydroxydetomidine
(metabolite)
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Detomidine
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HD-2001
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Hydroxydetomidine-d4
(deuterated metabolite)
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Furosemide
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FD-3010
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Furosemide-d5
(deuterated standard)
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Flunixin
|
zzz
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Flunixin-d3
(deuterated standard)
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Guafenesin
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GD-2551 |
Guafenesin-d4
(deuterated standard )
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ITPP
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I-9000
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(ITPP) myoInositolTrisPyroPhosphate
(standard)
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Ketoprofen
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KD-5025
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Ketoprofen-d3
(deuterated standard )
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Lidocaine
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H-2120
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3-Hydroxylidocaine
HCl
(metabolite)
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Lidocaine
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H-4520
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4-hydroxylidocaine HCl
(metabolite)
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Mazindol
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bbb
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2-(2-Aminoethyl)-3-(4-Chlorophenyl-3-Hydroxy-2,3-dihydro-isoindol-1-one
(metabolite)
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Mepivacaine
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H-5200
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4-Hydroxymepivacaine
(metabolite)
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Mepivacaine
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HD-2110
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3-Hydroxymepivacaine-d3
(deuterated metabolite)
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Mepivacaine
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H-5410
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4-Hydroxymepivacaine-d3
(deuterated metabolite)
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Methocarbamol
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ME-4501
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Methocarbamol-d4
(deuterated standard )
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methylprednisolone
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D-8500
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20-dihydro-6-methylprednisone
(isomeric metabolite of methylprednisolone standard)) |
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Modafinil
acid
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zzz
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Modafinil
acid
(metabolite standard)
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Phenylbutazone
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PD-0010
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Phenylbutazone-d9
(deuterated internal standard)
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Procaine
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PD-8210
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Procaine-d10
(deuterated standard )
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Promazine
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hypromazine
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3-Hydroxypromazine
(metabolite)
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Promethazine
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PMZS
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Promethazine
sulfoxide
(metabolite)
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Propanolol
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Hprop
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4-Hydroxypropanolol
(metabolite)
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undecylenic acid
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UnDcylAcid
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undecylenic acid (fragment of boldenone
undecylenate.
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Xylazine
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H-3100
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4-Hydroxyxylazine
(metabolite)
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