EQUINE MEDICATION AND METABOLITE REFERENCE 
STANDARDS AND STABLE ISOTOPE STANDARDS†


Sucheta Kudrimoti, Julio Gutierrez, Wojtek Karpiesiuk, Elizabeth Armstong, 
Charlie Hughes, Job Tharappel, and Thomas Tobin.
The Maxwell H. Gluck Equine Research Center, 
Department of Veterinary Science, 
University of Kentucky, Lexington, KY 40546

Rodney Eisenberg. 
Frontier Biopharm, PO Box 614, Richmond, KY 40476
www.frontierbiopharm.com

Brent Mayer and Emilie Stanley.  
Neogen Corp., Nandino Boulevard, Lexington, KY 40511 


The equine medication metabolite standards and stable isotope reference standards listed here were synthesized by a team of scientists with headquarters in the University of Kentucky Gluck Equine Research Center. These syntheses have been an ongoing project, starting in the mid-nineties when the need was recognized for standards of unique equine drug metabolites. More recently, there has been an increasing need for high-quality certified reference standards and stable isotope internal standards for use in quantitative equine medication regulation, or more simply, to allow the regulatory application of thresholds for therapeutic medications.
  
The University of Kentucky and Frontier Biopharm, in cooperation with Neogen Corp., are working to provide the racehorse drug testing community with certified quantitative analytical reference standards for equine therapeutic medications that will satisfy the ISO-17025 and ISO-34 requirements for quantitative measurements, and related reference materials which are increasingly required for equine forensic testing. These reference standards will meet industry requirements for chemical identity and purity in accordance with accepted ISO analytical criteria.
   
Synthesis of these standards has been made possible by ongoing research support from a large number of Horsemenís Benevolent and Protective Associationís, starting with the Florida HBPA in the mid-90s, and since then including the National Horsemenís Benevolent and Protective Association and the Alabama, Arizona, Arkansas, Canada, Charles Town West Virginia, Florida, Iowa, Indiana, Kentucky, Louisiana, Michigan, Minnesota, Nebraska, Ohio, Oklahoma, Ontario Canada, Oregon, Pennsylvania, Tampa Bay Downs Florida, Texas, Washington State, and West Virginia Horsemenís Benevolent and Protective Associations, the Kentucky Equine Drug Research Council, and the Kentucky Racing Commission, the Kentucky Horse Racing Authority and the Kentucky Science and Engineering Foundation, Grant agreement KSEF 148-502-05-160 with the Kentucky Science and Technology Corporation, and this support is gratefully acknowledged, along with the ongoing support of the Director and Faculty of the Maxwell H. Gluck Equine Research Center and the University of Kentucky College of Agriculture.†

  
  

Available Standards        --- follow the links to view PDF certificates of analysis ---

Parent Medication Product
Number
Regulatory Analyte or 
Deuterated Internal Standard

Acepromazine

H-1002

(HEPS-d4)   2-(1-hydroxyethyl) promazine sulfoxide-d4 maleate
(deuterated metabolite)

Acepromazine

H-1001

(HEPS)  2-(1-hydroxyethyl) promazine sulfoxide maleate
(metabolite)

Bupivacaine

BU-2950

3-Hydroxybupivacaine
(metabolite)

Clenbuterol

Clen-009

Clenbuterol-d9
(deuterated standard)

Detomidine

C-1001

Carboxydetomidine
(metabolite)

Detomidine

C-5001

3-Carboxydetomidine-d4
(deuterated metabolite)

Detomidine

H-2120

Hydroxydetomidine
(metabolite)

Detomidine

HD-2001

Hydroxydetomidine-d4
(deuterated metabolite)

Furosemide

FD-3010

Furosemide-d5
(deuterated standard)

Flunixin

zzz

Flunixin-d3
(deuterated standard)

Guafenesin

GD-2551

Guafenesin-d4
(deuterated standard )

ITPP I-9000 (ITPP)  myoInositolTrisPyroPhosphate (standard)

Ketoprofen

KD-5025

Ketoprofen-d3
(deuterated standard )

Lidocaine

H-2120

3-Hydroxylidocaine HCl
(metabolite)

Lidocaine   H-4520 4-hydroxylidocaine HCl
(metabolite)

Mazindol

bbb

2-(2-Aminoethyl)-3-(4-Chlorophenyl-3-Hydroxy-2,3-dihydro-isoindol-1-one
(metabolite)

Mepivacaine

H-5200

4-Hydroxymepivacaine
(metabolite)

Mepivacaine HD-2110 3-Hydroxymepivacaine-d3
(deuterated metabolite)
Mepivacaine H-5410 4-Hydroxymepivacaine-d3
(deuterated metabolite)

Methocarbamol  

ME-4501

Methocarbamol-d4
(deuterated standard )

methylprednisolone

D-8500

20-dihydro-6-methylprednisone  
(isomeric metabolite of methylprednisolone standard))

Modafinil acid

zzz

Modafinil acid
(metabolite standard)

Phenylbutazone

PD-0010

Phenylbutazone-d9
(deuterated internal standard)

Procaine 

PD-8210

Procaine-d10
(deuterated  standard )

Promazine

hypromazine

3-Hydroxypromazine
(metabolite)

Promethazine

PMZS

Promethazine sulfoxide
(metabolite)

Propanolol

Hprop

4-Hydroxypropanolol
(metabolite)

undecylenic acid UnDcylAcid undecylenic acid (fragment of boldenone undecylenate.
Xylazine H-3100 4-Hydroxyxylazine
(metabolite)